An exciting development at RARAF is the ability to irradiate Zebrafish and Medaka embryos at several different stages of development.
Studies on cardiac morphogenesis using the zebrafish embryo model
at the Columbia microbeam. The left image shows a 4X view taken
at RARAF of the outline of a 52 h post fertilization zebrafish
embryo whose heart is highlighted in the yellow rectangle. As
shown on the right (40X view), the cells of the atrium and the
ventriculum are green fluorescent protein (GFP)- or red fluorescent
protein (RFP)-tagged, respectively. In the contest of cardiac
regeneration studies, the function of a specific region of the
heart (e.g. atrium) can be determind upon microbeam irradiation
of another region of the heart (e.g. ventriculum).
Medaka embryos are irradiated through the corian using our proton beams. The embryos are oriented with respect to the eye pigmentation when it becomes visible.
Fluorescent z-stack image of the heart of a live zebrafish embryo carrying the transgene Tg(-5.1my17:nDsRed2)f2 (ZDB-ALT-060821-6). Cardiomyocyte nuclei in this strain express RFP which gives rise to the red spotted heart image and is useful for identifying single cells as potential microbeam targets. This image stack was acquired on the RARAF microbeam endstation and then deconvoluted using AutoQuant software.