7^th International Workshop:
 

Microbeam Probes of
Cellular Radiation Response

held March 15-17, 2006 at Columbia University in New York City

• Proceedings Publication

• Scientific Program

• Scientific Program Committee

The International Workshop on Microbeam Probes of Cellular Radiation Response has been held regularly since 1993. In the past, these workshops have been highly successful in bringing together groups interested in developing and applying micro-irradiation techniques to the study of cell and tissue damage by ionizing radiations.

There is continuing interest in the use of microbeam systems, which can deliver beams of different radiations with a spatial resolution of a few micrometers or less, for radiobiological research. Single-particle or photon microbeams can be used to address such questions as the effects of irradiation on non-hit neighboring (bystander) cells, communication of damage information, and the relative sensitivities of different parts of the cell (e.g. nucleus vs. cytoplasm). For particle microbeams, irradiation with exactly one (or more) particle per cell can be achieved, allowing questions of risks from very low doses of ionizing radiations to be addressed. Several microbeams are now in operation, and others are being developed. This Workshop will provide a forum to assess the current state of microbeam technology and current biological applications, and to discuss future directions for development, both technological and biological.

• The Workshop was organized by Columbia University's Radiological Research Accelerator Facility.

• The Workshop was held at Columbia University's Kellogg Center in New York City.

• Registration was free.

• A maximum $750 travel award was paid as reimbursement for travel expenses to many participants.

Support for this workshop was provided by National Institutes of Health grant P41 EB002033-10 and U.S. Department of Energy Low Dose Radiation Program grant DE-FG02-06ER64217.

 

PUBLICATION INFORMATION FOR AUTHORS

Thanks to all authors who submitted abstracts.

The extended abstracts of the meeting were published in Radiation Research: Proceedings of the 7^th International Workshop: Microbeam Probes of Cellular Radiation Response published in Radiation Research (166: 652-689, 2006).

 

SCIENTIFIC PROGRAM

The Microbeam Workshop broadly followed the same format as previous meetings and covered all aspects of the development and application of micro-irradiation techniques as a method for inflicting highly localized radiation damage to living cells and tissues. The meeting was structured around poster sessions, which allowed for optimal interpersonal communication and information transfer.  Short oral presentations, with time for discussion, introduced the poster presentations. Further detailed discussions were then at the subsequent, more leisurely, poster session.

The agenda for the meeting was as follows:

Agenda in PDF

(* indicates oral mini-presentation)

Wednesday March 15

9:30 - 2:00 (optional)		Informal open house at the Radiological Research Accelerator Facility (RARAF)
4:00 - 5:00		Registration & Poster Hanging
5:00 - 6:00		Cheese and wine reception
6:00 - 6:45		Introductory Lecture: William M. Bonner, Ph.D., National Cancer Institute, "γ-H2AX"

 

Thursday March 16

9:00	-	9:20		Welcome & Introduction
9:20	-	10:00		Session 1: Microbeam Source Development Chair: Melvyn Folkard *Christoph Greubel (University of Munich, Germany) The Munich Microprobe SNAKE, a Single-Ion Cell Irradiation Facility *Karen Kirkby (University of Surrey, UK) A Scanning Focussed Vertical Ion Nanobeam: A New UK Facility for Cell Irradiation and Analysis *Natalia Arteaga Marrero (LTH, Lund University, Sweden) Development of the Lund Irradiation Facility at the New Sub-Micron Beam Line *Guy Garty (Columbia University) Status of the Stand-Alone Microbeam at Columbia University
10:00	-	10:30		Coffee
10:30	-	11:50		Session 2: Photon/Electron Microbeams Chair: Marianne Sowa *Paul Blackborrow (Energetiq, Inc.) A Novel, High-Brightness Electrodeless Z-Pinch Soft X-Ray Source for Microbeam Applications *Stanley Botchway (CCLRC, Rutherford Appleton Laboratory, UK) Femtosecond Near Infra-red Laser Microbeam Technique for Sub-Micron Point Source for High-Resolution Cell DNA Damage, Signalling and Repair Studies *Sha Chang (UNC School of Medicine) Development of a carbon nanotube based low LET multi-pixel microbeam array *Melvyn Folkard (Gray Cancer Institute, UK) Development of the GCI Multi-target X-Ray Microprobe *Giselle Flaccavento (Gray Cancer Institute, Japan) Performance of the GCI Multi-target X-ray Microprobe *Katsumi Kobayashi (KEK, Japan) X-ray microbeam irradiation systems at the Photon Factory - Present Status and Future Plan *Giuseppe Schettino (Columbia University) Development of an soft X-ray microbeam at RARAF *Noriko Usami (KEK, Japan) Biological Experiments Visualizing Radiation-Induced Responses with Synchrotron X-Ray Microbeam at the Photon Factory
11:50	-	12:50		Poster Viewing, Sessions 1 & 2
12:50	-	2:00		Lunch (complementary for registered participants)
2:00	-	2:50		Session 3: Microbeam Endstations Chair: Gerhard Randers-Pehrson *Borivoj Vojnovic (Gray Cancer Institute, UK) The GCI Multi-target X-Ray Microprobe: Imaging, Control and Software Automation *Alan Bigelow (Columbia University) Multiphoton microscope design for the Columbia University Microbeam II endstation *Silvia Gerardi (INFN-Laboratori Nazionali di Legnaro, Italy) Ion beam quality assessment at the INFN-LNL single-ion microbeam facility. A combined approach: simulations and measurements *Wojciech Polak (University of Surrey, UK) Progress Report on the IFJ PAN Microprobe for Living Cells Irradiations: Technical Setup and First Experimental Data Thomas Pouthier (CENBG, Bordeaux, France) Cellular geometry modelling for Monte Carlo microdosimetry *Greg Ross (Columbia University) Update on incorporating no-stain imaging into microbeam endstation
2:50	-	3:20		Tea
3:20	-	4:00		Session 4: Microbeam Status Reports Chair: Marian Cholewa *Anna Friedl (University of Munich, Germany) Radiobiological Experiments at the Munich Microprobe SNAKE Klaus-Dieter Greif (PTB, Germany) New Capabilities and Experimental Programme of the PTB Microbeam *Markus Heiß (GSI, Germany) Update and performance of the scanning ion microprobe at GSI *Hicham Khodja (Commissariat à l'Energie Atomique, France) The LPS Saclay Single Ion Microbeam Facility Yasuhiko Kobayashi (Japan Atomic Energy Agency (JAEA-Takasaki)) Current status of Heavy-Ion Microbeams at JAEA-Takasaki *Charlotta Nilsson (University of Leipzig, Germany) Status of the new single ion hit facility for irradiation of living cells at LIPSION Hervé Seznec (CENBG, Bordeaux, France) Biological validation of the ion-microbeam developed at CENBG to generate localized ionizing radiation induced-damage on keratinocyte cell line expressing GFP-tagged protein
4:00	-	5:00		Poster Viewing, Sessions 3 & 4
				Dinner (on your own)

 

Friday March 17

9:00	-	9:10		Introduction
9:10	-	9:50		Session 5: DNA Repair Probed with Microbeams Chair: William F. Morgan *Anja Fiedler (University of Leipzig, Germany) DNA-DSBs and Hsp70 expression in proton irradiated living cells at LIPSION *Tomoo Funayama (Japan Atomic Energy Agency (JAEA-Takasaki)) Effect of Heavy-ion Irradiation on Phosphorylation of Histone H2AX *Volker Hable (University of Munich, Germany) Dynamics of DNA-repair proteins after directed heavy ion cell irradiation Sebastien Incerti (CENBG, Bordeaux, France) Monte Carlo simulation of interactions of radiation with biological systems at the cellular and DNA levels: the Geant4-DNA project Alex Lyakhovich (Universitat Autonoma de Barcelona, Spain) Study the dynamics and regulation of Fanconi proteins through microbeam technique Maddalena Moganto (Università di Padova, Italy) Repair of double strand breaks induced by low-energy protons and X-rays in human G0 lymphocytes cultured in 1g and in modeled microgravity *Naoya Uematsu (UT Southwestern) Dynamics of DSB sensing and repair proteins at DNA-double stand breaks in living cells
10:00	-	10:30		Coffee
10:30	-	11:00		Session 6: Cellular Response Probed with Microbeams Chair: Michael Cornforth Denise Macdonald (Medical Research Council, Harwell, UK) Inter- and intra-individual differences in genomic instability after targeted microbeam irradiation. *Munetoshi Maeda (KEK, Japan) Cell Survival Study Using KEK-PF Synchrotron X-ray Microbeam Irradiation System *Ivan Onishchenko (Kharkov Medical University, Ukraine) Micronucleus Expression in Peripheral Lymphocytes Cultivated with Activated Cisplatin *Morgiane Richard (University of Surrey, UK) A Mathematical Model of Cellular Behaviour under Irradiation
11:00	-	12:00		Poster Viewing, Sessions 5 & 6
12:00	-	1:10		Lunch (complementary for registered participants)
1:10	-	2:20		Session 7: Bystander Responses Chair: Edouard Azzam Ferya Banaz-Yasar (Inst. of Anatomy Univ. Hosp. Essen, Germany) Evaluation of the role of gap junctional communication in mediating radiation-induced bystander effects in Jeg3 trophoblast cells *Philippe Barberet (GSI, Germany) Studies on the induction of the cell cycle regulator CDKN1A (p21) in bystander cells using the GSI heavy ion microbeam *Eleanor Blakely (Lawrence Berkeley National Laboratory) Radiation-Induced Bystander Effects with a 12.5 keV X-Ray Microbeam Nobuyuki Hamada (Gunma University Graduate School of Medicine, Japan) Bystander Killing in Normal Human Fibroblasts by Heavy-Ion Microbeams *John Miller (Washington State University) Is the Plasma Membrane a Target for Generation of Signals to Bystanders? *Brian Ponnaiya (Columbia University) Comparisons of bystander responses observed in 2-dimension versus 3-dimension systems following microbeam irradiation Emma Pyke (Medical Research Council, Harwell, UK) Tracking Down The Bystander Response Lori Rastogi (Bhabha Atomic Research Centre, India) Modification in Cellular Bystander Response in Lymphocytes and Lung Carcinoma by Extra-cellular Diffusible Factors from Irradiated Cultures *Olga Sedelnikova (NIH) Long-term biological bystander effects of microbeam irradiation in 3D human tissue models *Marianne Sowa (Pacific Northwest National Laboratory) Low LET Bystander Responses in Gap Junction Null Human Colon Carcinoma Cells (RKO36) *Hongning Zhou (Columbia University) Cytoplasmic Irradiation Induced Bystander Mutagenesis
2:20	-	3:20		Poster Viewing, Session 7
3:20	-	4:20		Round Table Discussion: Where should we go with microbeams? Chair: Eric J. Hall
				Departure

 

SCIENTIFIC PROGRAM COMMITTEE

Leslie Braby (Texas A&M, College Station, TX)
David Brenner (Columbia University, New York, NY)
Roberto Cherubini (INFN, Legnaro, Italy)
Thomas Cremer (Ludwig-Maximilians-Universität, Munich, Germany)
Marco Durante (INFN, Napoli, Italy)
Charles Geard (Columbia University, New York, NY)
Katsumi Kobayashi (KEK, Tsukuba, Japan)
John Miller (Washington State University, Richland, WA)
Kevin Prise (Gray Cancer Institute, London, England)
Gisela Taucher-Scholz (GSI, Darmstadt, Germany)

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For additional information contact David Brenner.
Last modified by CE on January 29, 2009.