Multi-Photon Microscope
Integrated on the
Microbeam II End Station at RARAF
RARAF microbeam users are increasingly using and planning to use 3-D tissue
samples and small organisms. To image their 3-D samples, our users require the
multiphoton microscope that we developed and fully integrated into our main
electrostatic-lens Microbeam II endstation at RARAF. A custom-design for the
multiphoton microscope was necessary, given the geometrical constraints of the
pre-existing microscope fitted at the terminus of the vertical ion beamline. Intended
for detecting and observing short-term molecular kinetics of radiation responses in
living tissue and in cell-culture samples, the multiphoton microscope at RARAF is the
first of its kind to be assembled and implemented onto a microbeam cell-irradiation platform.
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