Flow And Shoot Technology
(FAST) integrates the existing RARAF microbeam technology
with microfluidics tools. Instead of irradiating cells
attached to a thin film, the cells are confined to a channel
thinner than the range of the particles and as wide as
the field of view of the imaging and beam targeting system.
As the cells flow through the channel, they are continuously
tracked and targeted using the point & shoot system,
as shown in the movie.
This allows microbeam irradiation of lymphocytes and
other cells that cannot be attached to a surface. It also
allows, coupling the microbeam to microfluidic devices
for cell sorting and analysis.